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promoterless luciferase expression vector pgl3-basic  (Promega)

 
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    Structured Review

    Promega promoterless luciferase expression vector pgl3-basic
    Promoterless Luciferase Expression Vector Pgl3 Basic, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/promoterless+luciferase+expression+vector+pgl3-basic/pgl3+basic/pm35302184-60-63-68
    Average 90 stars, based on 1 article reviews
    promoterless luciferase expression vector pgl3-basic - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: Gastric Mammalian Target of Rapamycin Signaling Regulates Ghrelin Production and Food Intake
    Article Snippet: Construction of human ghrelin promoter-luciferase expression vectors A 3143-bp segment of the 5′-upstream regulatory region of the human ghrelin gene (−3161/−18, GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"NC_000003.10","term_id":"89161205","term_text":"NC_000003.10"}} NC_000003.10 ) was cloned by PCR amplification using human genomic DNA (BD Biosciences) (forward primer, 5′-AGGTGGCAGGCCCTCAAGAGACCCGCAACAGA-3′; reverse primer, 5′-AGACAGGTGGGCCTGGGGGAGAGAGGGTCT-3′) and subcloned into promoterless luciferase expression vector pGL3-Basic (Promega).

    Article Title: Gastric Mammalian Target of Rapamycin Signaling Regulates Ghrelin Production and Food Intake
    Article Snippet: A 3143-bp segment of the 5′-upstream regulatory region of the human ghrelin gene (−3161/−18, GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"NC_000003.10","term_id":"89161205","term_text":"NC_000003.10"}} NC_000003.10 ) was cloned by PCR amplification using human genomic DNA (BD Biosciences) (forward primer, 5′-AGGTGGCAGGCCCTCAAGAGACCCGCAACAGA-3′; reverse primer, 5′-AGACAGGTGGGCCTGGGGGAGAGAGGGTCT-3′) and subcloned into promoterless luciferase expression vector pGL3-Basic (Promega).

    Article Title: DOCK4 regulates ghrelin production in gastric X/A-like cells.
    Article Snippet: Purpose Ghrelin, a gastric hormone, provides a hunger signal to the central nervous system to stimulate food intake.. Ghrelin also modulates neuroinflammatory and apoptotic processes.. Dedicator of cytokinesis 4 (DOCK4), a guanine nucleotide exchange factor (GEF), is involved in the regulation of neuronal polarization and axon regeneration.

    Clone Assay:

    Article Title: Gastric Mammalian Target of Rapamycin Signaling Regulates Ghrelin Production and Food Intake
    Article Snippet: Construction of human ghrelin promoter-luciferase expression vectors A 3143-bp segment of the 5′-upstream regulatory region of the human ghrelin gene (−3161/−18, GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"NC_000003.10","term_id":"89161205","term_text":"NC_000003.10"}} NC_000003.10 ) was cloned by PCR amplification using human genomic DNA (BD Biosciences) (forward primer, 5′-AGGTGGCAGGCCCTCAAGAGACCCGCAACAGA-3′; reverse primer, 5′-AGACAGGTGGGCCTGGGGGAGAGAGGGTCT-3′) and subcloned into promoterless luciferase expression vector pGL3-Basic (Promega).

    Article Title: Gastric Mammalian Target of Rapamycin Signaling Regulates Ghrelin Production and Food Intake
    Article Snippet: A 3143-bp segment of the 5′-upstream regulatory region of the human ghrelin gene (−3161/−18, GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"NC_000003.10","term_id":"89161205","term_text":"NC_000003.10"}} NC_000003.10 ) was cloned by PCR amplification using human genomic DNA (BD Biosciences) (forward primer, 5′-AGGTGGCAGGCCCTCAAGAGACCCGCAACAGA-3′; reverse primer, 5′-AGACAGGTGGGCCTGGGGGAGAGAGGGTCT-3′) and subcloned into promoterless luciferase expression vector pGL3-Basic (Promega).

    Article Title: DOCK4 regulates ghrelin production in gastric X/A-like cells.
    Article Snippet: Purpose Ghrelin, a gastric hormone, provides a hunger signal to the central nervous system to stimulate food intake.. Ghrelin also modulates neuroinflammatory and apoptotic processes.. Dedicator of cytokinesis 4 (DOCK4), a guanine nucleotide exchange factor (GEF), is involved in the regulation of neuronal polarization and axon regeneration.

    Polymerase Chain Reaction:

    Article Title: Gastric Mammalian Target of Rapamycin Signaling Regulates Ghrelin Production and Food Intake
    Article Snippet: Construction of human ghrelin promoter-luciferase expression vectors A 3143-bp segment of the 5′-upstream regulatory region of the human ghrelin gene (−3161/−18, GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"NC_000003.10","term_id":"89161205","term_text":"NC_000003.10"}} NC_000003.10 ) was cloned by PCR amplification using human genomic DNA (BD Biosciences) (forward primer, 5′-AGGTGGCAGGCCCTCAAGAGACCCGCAACAGA-3′; reverse primer, 5′-AGACAGGTGGGCCTGGGGGAGAGAGGGTCT-3′) and subcloned into promoterless luciferase expression vector pGL3-Basic (Promega).

    Article Title: Gastric Mammalian Target of Rapamycin Signaling Regulates Ghrelin Production and Food Intake
    Article Snippet: A 3143-bp segment of the 5′-upstream regulatory region of the human ghrelin gene (−3161/−18, GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"NC_000003.10","term_id":"89161205","term_text":"NC_000003.10"}} NC_000003.10 ) was cloned by PCR amplification using human genomic DNA (BD Biosciences) (forward primer, 5′-AGGTGGCAGGCCCTCAAGAGACCCGCAACAGA-3′; reverse primer, 5′-AGACAGGTGGGCCTGGGGGAGAGAGGGTCT-3′) and subcloned into promoterless luciferase expression vector pGL3-Basic (Promega).

    Article Title: DOCK4 regulates ghrelin production in gastric X/A-like cells.
    Article Snippet: Purpose Ghrelin, a gastric hormone, provides a hunger signal to the central nervous system to stimulate food intake.. Ghrelin also modulates neuroinflammatory and apoptotic processes.. Dedicator of cytokinesis 4 (DOCK4), a guanine nucleotide exchange factor (GEF), is involved in the regulation of neuronal polarization and axon regeneration.

    Amplification:

    Article Title: Gastric Mammalian Target of Rapamycin Signaling Regulates Ghrelin Production and Food Intake
    Article Snippet: Construction of human ghrelin promoter-luciferase expression vectors A 3143-bp segment of the 5′-upstream regulatory region of the human ghrelin gene (−3161/−18, GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"NC_000003.10","term_id":"89161205","term_text":"NC_000003.10"}} NC_000003.10 ) was cloned by PCR amplification using human genomic DNA (BD Biosciences) (forward primer, 5′-AGGTGGCAGGCCCTCAAGAGACCCGCAACAGA-3′; reverse primer, 5′-AGACAGGTGGGCCTGGGGGAGAGAGGGTCT-3′) and subcloned into promoterless luciferase expression vector pGL3-Basic (Promega).

    Article Title: Gastric Mammalian Target of Rapamycin Signaling Regulates Ghrelin Production and Food Intake
    Article Snippet: A 3143-bp segment of the 5′-upstream regulatory region of the human ghrelin gene (−3161/−18, GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"NC_000003.10","term_id":"89161205","term_text":"NC_000003.10"}} NC_000003.10 ) was cloned by PCR amplification using human genomic DNA (BD Biosciences) (forward primer, 5′-AGGTGGCAGGCCCTCAAGAGACCCGCAACAGA-3′; reverse primer, 5′-AGACAGGTGGGCCTGGGGGAGAGAGGGTCT-3′) and subcloned into promoterless luciferase expression vector pGL3-Basic (Promega).

    Article Title: DOCK4 regulates ghrelin production in gastric X/A-like cells.
    Article Snippet: Purpose Ghrelin, a gastric hormone, provides a hunger signal to the central nervous system to stimulate food intake.. Ghrelin also modulates neuroinflammatory and apoptotic processes.. Dedicator of cytokinesis 4 (DOCK4), a guanine nucleotide exchange factor (GEF), is involved in the regulation of neuronal polarization and axon regeneration.

    Countercurrent Chromatography:

    Article Title: Gastric Mammalian Target of Rapamycin Signaling Regulates Ghrelin Production and Food Intake
    Article Snippet: Construction of human ghrelin promoter-luciferase expression vectors A 3143-bp segment of the 5′-upstream regulatory region of the human ghrelin gene (−3161/−18, GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"NC_000003.10","term_id":"89161205","term_text":"NC_000003.10"}} NC_000003.10 ) was cloned by PCR amplification using human genomic DNA (BD Biosciences) (forward primer, 5′-AGGTGGCAGGCCCTCAAGAGACCCGCAACAGA-3′; reverse primer, 5′-AGACAGGTGGGCCTGGGGGAGAGAGGGTCT-3′) and subcloned into promoterless luciferase expression vector pGL3-Basic (Promega).

    Article Title: Gastric Mammalian Target of Rapamycin Signaling Regulates Ghrelin Production and Food Intake
    Article Snippet: A 3143-bp segment of the 5′-upstream regulatory region of the human ghrelin gene (−3161/−18, GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"NC_000003.10","term_id":"89161205","term_text":"NC_000003.10"}} NC_000003.10 ) was cloned by PCR amplification using human genomic DNA (BD Biosciences) (forward primer, 5′-AGGTGGCAGGCCCTCAAGAGACCCGCAACAGA-3′; reverse primer, 5′-AGACAGGTGGGCCTGGGGGAGAGAGGGTCT-3′) and subcloned into promoterless luciferase expression vector pGL3-Basic (Promega).

    Article Title: DOCK4 regulates ghrelin production in gastric X/A-like cells.
    Article Snippet: Purpose Ghrelin, a gastric hormone, provides a hunger signal to the central nervous system to stimulate food intake.. Ghrelin also modulates neuroinflammatory and apoptotic processes.. Dedicator of cytokinesis 4 (DOCK4), a guanine nucleotide exchange factor (GEF), is involved in the regulation of neuronal polarization and axon regeneration.

    Luciferase:

    Article Title: Gastric Mammalian Target of Rapamycin Signaling Regulates Ghrelin Production and Food Intake
    Article Snippet: Construction of human ghrelin promoter-luciferase expression vectors A 3143-bp segment of the 5′-upstream regulatory region of the human ghrelin gene (−3161/−18, GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"NC_000003.10","term_id":"89161205","term_text":"NC_000003.10"}} NC_000003.10 ) was cloned by PCR amplification using human genomic DNA (BD Biosciences) (forward primer, 5′-AGGTGGCAGGCCCTCAAGAGACCCGCAACAGA-3′; reverse primer, 5′-AGACAGGTGGGCCTGGGGGAGAGAGGGTCT-3′) and subcloned into promoterless luciferase expression vector pGL3-Basic (Promega).

    Article Title: Gastric Mammalian Target of Rapamycin Signaling Regulates Ghrelin Production and Food Intake
    Article Snippet: A 3143-bp segment of the 5′-upstream regulatory region of the human ghrelin gene (−3161/−18, GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"NC_000003.10","term_id":"89161205","term_text":"NC_000003.10"}} NC_000003.10 ) was cloned by PCR amplification using human genomic DNA (BD Biosciences) (forward primer, 5′-AGGTGGCAGGCCCTCAAGAGACCCGCAACAGA-3′; reverse primer, 5′-AGACAGGTGGGCCTGGGGGAGAGAGGGTCT-3′) and subcloned into promoterless luciferase expression vector pGL3-Basic (Promega).

    Article Title: DOCK4 regulates ghrelin production in gastric X/A-like cells.
    Article Snippet: Purpose Ghrelin, a gastric hormone, provides a hunger signal to the central nervous system to stimulate food intake.. Ghrelin also modulates neuroinflammatory and apoptotic processes.. Dedicator of cytokinesis 4 (DOCK4), a guanine nucleotide exchange factor (GEF), is involved in the regulation of neuronal polarization and axon regeneration.

    Plasmid Preparation:

    Article Title: Gastric Mammalian Target of Rapamycin Signaling Regulates Ghrelin Production and Food Intake
    Article Snippet: Construction of human ghrelin promoter-luciferase expression vectors A 3143-bp segment of the 5′-upstream regulatory region of the human ghrelin gene (−3161/−18, GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"NC_000003.10","term_id":"89161205","term_text":"NC_000003.10"}} NC_000003.10 ) was cloned by PCR amplification using human genomic DNA (BD Biosciences) (forward primer, 5′-AGGTGGCAGGCCCTCAAGAGACCCGCAACAGA-3′; reverse primer, 5′-AGACAGGTGGGCCTGGGGGAGAGAGGGTCT-3′) and subcloned into promoterless luciferase expression vector pGL3-Basic (Promega).

    Article Title: Gastric Mammalian Target of Rapamycin Signaling Regulates Ghrelin Production and Food Intake
    Article Snippet: A 3143-bp segment of the 5′-upstream regulatory region of the human ghrelin gene (−3161/−18, GenBank accession No. {"type":"entrez-nucleotide","attrs":{"text":"NC_000003.10","term_id":"89161205","term_text":"NC_000003.10"}} NC_000003.10 ) was cloned by PCR amplification using human genomic DNA (BD Biosciences) (forward primer, 5′-AGGTGGCAGGCCCTCAAGAGACCCGCAACAGA-3′; reverse primer, 5′-AGACAGGTGGGCCTGGGGGAGAGAGGGTCT-3′) and subcloned into promoterless luciferase expression vector pGL3-Basic (Promega).

    Article Title: DOCK4 regulates ghrelin production in gastric X/A-like cells.
    Article Snippet: Purpose Ghrelin, a gastric hormone, provides a hunger signal to the central nervous system to stimulate food intake.. Ghrelin also modulates neuroinflammatory and apoptotic processes.. Dedicator of cytokinesis 4 (DOCK4), a guanine nucleotide exchange factor (GEF), is involved in the regulation of neuronal polarization and axon regeneration.



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